Please complete the form below. Create a Sequencing Order Select A ServiceSelect an optionReady-to-load PlateFull ServicePlease see our "Services" page if you are unsure which service is best for you.Enter Your Name*Enter Your E-mail Address*Enter A Plate Name*Names_must_not_contain_spaces.Would you like custom data labels?Select an optionYesNoIf you select "no" your data will be sent with the well position as a label.Please fill in the excel sheet with your custom labels and email to angelas@uwm.edu. Download Data Entry SheetHow are you sending your plates?Select an optionPostUWM Intercampus MailUWM SFS ShuttleHow Many Reactions Are Needed?Select an optionLess than 4848 or moreWhat is your template?Select an optionPlasmid DNAPCR ProductWhat is your template?Select an optionPlasmid DNAPCR ProductWhen sending 48 or more samples please send them in a 96 well plate. 300-600ng of template is needed in a volume of 1-6uL. Include 0.8uL of primer in each well. Please be sure to use plates compatible with an ABI 3730 DNA Analyzer. We recommend these: Dot Scientific, catalog # 951-PCR. If sending a half-plate (48 samples), please only use columns 1, 3, 5, 7, 9, and 11. Download and complete the excel file below and email to angelas@uwm.edu. Please note the volume of template in each well. If sample names are left blank, the data you receive will be labeled by well position Download Data Entry Sheet When sending 48 or more samples please send them in a 96 well plate. 10-20ng of template is needed in a volume of 1-6uL. Include 0.8uL of primer in each well. Please be sure to use plates compatible with an ABI 3730 DNA Analyzer. We recommend these: Dot Scientific, catalog # 951-PCR. If sending a half-plate (48 samples), please only use columns 1, 3, 5, 7, 9, and 11. Download and complete the excel file below and email to angelas@uwm.edu. Please note the volume of template in each well. If sample names are left blank, the data you receive will be labeled by well position Download Data Entry Sheet Please send your samples in 1.5 mL microcentrifuge tubes with a label no more than 3 digits in length. Prepare your samples to a volume of at least 10uL and a concentration of 100-200 ng/uL See our Services page for details on sample preparation Please send your samples in 1.5 mL microcentrifuge tubes with a label no more than 3 digits in length. Prepare your samples to a volume of at least 10μL and a concentration of 3-8 ng/μL. Include 1μL of primer per reaction at a concentration of 10-20pmol See our Services page for details on sample preparation Click the link below to download an excel file to enter your tube labels and corresponding well position. If needed, you may add a sample name for the resulting data to be labeled. If the sample name is left blank your data will be named with its well position. If multiple primers are to be used indicate this on the excel file. Download Data Entry Sheet Upon completion, please email the excel file to angelas@uwm.eduHow are you sending your samples?Select an optionPostUWM Intercampus MailUWM SFS ShuttlePlease send your package(s) to the following address: Angela Schmoldt 600 E Greenfield Ave UWM School of Freshwater Sci Genomics Center Milwaukee, WI 53204Packages are delivered to SFS around noon. For samples to be delivered same day, make sure they are in a mailbag as early as possible. Please address your packages to: Genomics Center School of Freshwater SciencesTo send plates via SFS shuttle meet the shuttle driver outside a designated stop. Hand the driver the package and let the Genomics Center know what time the shuttle is expected to arrive at SFS. The schedule can be found at http://www4.uwm.edu/parking/ on the right-hand side of the page Send notification emails to angelas@uwm.edu
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