The Great Lakes Genomics Center will quantify and assess quality of DNA and RNA extractions using the Qubit Fluorometer, Agilent Bioanalyzer 2100, and Nanodrop.
The Qubit uses a fluorescence – based method to quantify nucleic acids that is not influenced by contaminants and is therefore one of the most accurate quantification methods available.
The Agilent Bioanalyzer 2100 is the industry standard for RNA quality assessment. A RIN (RNA integrity number) will be provided, based on the 16S/18S ratio.
The Nanodrop 1000 260/230 and 260/280 ratios will provide sample purity information.
RNA samples need to be shipped overnight on dry ice and DNA overnight on wet ice.